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Conflicts of interest statement
The authors declare that they have no conflicts of interests.
This study was supported by grants from the Ministry of Economy, Industry and Competitiveness, Government of Spain (SAF2014-56623-R and SAF2017-82689-R) and from the “Mutua Madrileña Investigación Biomédica” Foundation, Spain (FMM2011) to C. Montiel and F. Arnalich. J.L. Cedillo was recipient of a FPU fellowship (Ministry of Education, Culture and Sports, Government of Spain); A. Bordas and R. L. Arribas are recipients of a FPI fellowship (Autonomous University of Madrid); C. Martín-Sanchez was recipient of a FPI fellowship (Ministry of Economy, Industry and Competitiveness, Government of Spain). Lung Cancer 128 (2019) 134–144
Finally, M. Extremera and G. Atienza were hired with funds from the grants specified above. The authors thank Raquel Marcos-Hernández (Laboratory Technician) from Pathology Service, La Paz University Hospital, Madrid), for her help with the IHC experiments.
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Contents lists available at ScienceDirect
Journal of Magnetism and Magnetic Materials
journal homepage: www.elsevier.com/locate/jmmm
Antibody conjugated onto surface modified magnetic nanoparticles for separation of HER2+ breast cancer cells
Amir Hossein Haghighia, Zahra Faghihb, Mohammad Taghi Khorasanic, , Fatemeh Farjadiand
a Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
b Shiraz Institute for Cancer Research, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
c Biomaterials Department, Iran Polymer and Petrochemical Institute, Tehran, Iran
d Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
Breast cancer is one of the leading causes of death among women worldwide, therefore early diagnosis and monitoring the status of cancer are essential. In the present study we developed antibody-conjugated iron oxide magnetic nanoparticles (MNPs) with highly eﬃcient potential to detect HER2-expressing cancer cells in blood, a compartment where tumor cells are very rare. The MNPs were first coated with (3-aminopropyl) trimethox-ysilane. Eﬀective coating was checked by VSM, XRD, FT-IR, TGA, EDX, and SEM. Anti-HER2 antibody (Herceptin) was conjugated on the surface of silane-coated MNPs (MNP-Si). The aﬃnity of antibody-conjugated MNPs (Ab/MNP-Si) for HER2-expressing cells was evaluated in the SK-BR-3 cell line as a breast cancer cell model. Diﬀerent concentrations of SK-BR-3 cells were mixed with peripheral blood mononuclear cells and subjected to isolation with Ab/MNP-Si in a magnetic field. Separation eﬃciency, as determined by flow cyto-metry, was 77–98%. Further evaluation with fluorescence microscopy also confirmed the ability of Ab/MNP-Si to detect low numbers of SK-BR-3 cells in whole blood. Our results suggest that Ab/MNP-Si is a potentially useful tool to isolate circulating tumor cells by targeting tumor-specific surface receptors under a magnetic field, and may improve breast cancer diagnosis and monitoring.