U0126 Ordered from IDT mouseprimerdepot nci nih gov br
Ordered from IDT
mouseprimerdepot.nci.nih.gov
RORc REV: TGCAGGAGTAGGCCACATTACA
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL17F FW: CCTCCCCTGGAGGATAACAC
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL17F REV: CATGGGGAACTGGAGCGGTT
Ordered from IDT
mouseprimerdepot.nci.nih.gov
Ptgs2 (COX2) FW: TGAGCAACTATTCCAAACCAGC
Ordered from IDT
mouseprimerdepot.nci.nih.gov
Ptgs2 (COX2) REV: GCACGTAGTCTTCGATCACTATC
Ordered from IDT
mouseprimerdepot.nci.nih.gov
16S bacterial RNA FW: AGAGTTTGATCMTGGCTCAG
Ordered from IDT
16S bacterial RNA REV: TACGGYTACCTTGTTACGACTT
Ordered from IDT
SFB FW: GACGCTGAGGCATGAGAGCAT
Ordered from IDT
SFB REV: GACGGCACGGATTGTTATTCA
Ordered from IDT
E.Coli FW: U0126 CATGCCGCGTGTATGAAGAA
Ordered from IDT
E.Coli REV: CGGGTAACGTCAATGAGCAAA
Ordered from IDT
Enterobacteria FW: GTGCCAGCMGCCGCGGTAA
Ordered from IDT
Enterobacteria REV: GCCTCAAGGGCACAACCTCCAAG
Ordered from IDT
Clostridia FW: CGCATAACGTTGAAAGATGG
Ordered from IDT
Clostridia REV: CCTTGGTAGGCCGTTACCC
Ordered from IDT
Bacteroides FW: GGTTCTGAGAGGAGGTCCC
Ordered from IDT
Bacteroides REV: GCTGGCTCCCGTAGGAGT
Ordered from IDT
(Continued on next page)
Continued
REAGENT or RESOURCE
SOURCE
IDENTIFIER
S100A8 FW
Ordered from IDT
mouseprimerdepot.nci.nih.gov
CCAATTCTCTGAACAAGTTTTCG
S100A8 REV
Ordered from IDT
mouseprimerdepot.nci.nih.gov
TCACCATGCCCTCTACAAGA
S100A9 FW: GTCCAGGTCCTCCATGATGT
Ordered from IDT
mouseprimerdepot.nci.nih.gov
S100A9 REV: GAAGGAAGGACACCCTGACA
Ordered from IDT
mouseprimerdepot.nci.nih.gov
Reg3b FW: ACTCCCTGAAGAATATACCCTCC
Ordered from IDT
mouseprimerdepot.nci.nih.gov
Reg3b REV: CGCTATTGAGCACAGATACGAG
Ordered from IDT
mouseprimerdepot.nci.nih.gov
Reg3g FW: TGCTGCTCTCCTGCCTGATG
Ordered from IDT
mouseprimerdepot.nci.nih.gov
Reg3g REV: ATAGGAGCCATAGGCACGGG
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL33 FW: TCCAACTCCAAGATTTCCCCG
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL33 REV: CATGCAGTAGACATGGCAGAA
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL18 FW: TCCTTGAAGTTGACGCAAGA
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL18 REV: TCCAGCATCAGGACAAAGAA
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL1F10 FW: TGTCTGAATCAGGGTCTCCC
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL1F10 REV: TGAAGACCAGACACTCCCAA
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL36a FW: GGCTTTTACAGGTTCCTTTTTG
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL36a REV: GATGAGCTGCCTGTTCTGC
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL36g FW: GTCCGGGTGTGGTAAAACAG
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL36g REV: GGACACCCTACTTTGCTGCT
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL36b FW: AGAGTATTCAAATGTGGGAACCG
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL36b REV: GACCCATACCATCTGTTGTGAG
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL36Ra FW: GCAGCTGGTTATTGTGCAGAT
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL36Ra REV: TGGAGCTCATGATGGTTCTG
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL36R (IL1RL2) FW: TCCCCGCTTTAATCTCTTCA
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL36R (IL1RL2) REV: ATCGGGCAGTCTGAATTGTC
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL18bp FW: TCTCCAGCAGTCCCAACTAAGC
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL18bp REV: AGGCAGTACAGGACAAGGTCAG
Ordered from IDT
mouseprimerdepot.nci.nih.gov
IL1R exon5 FW: TGGAAGTCTTGTGTGCCCTT
Ordered from IDT
This paper
IL1R exon5 REV: ACTCCGAAGAAGCTCACGTT
Ordered from IDT
This paper
Software and Algorithms
Flow Jo 9.7.6
FlowJo LLC
https://www.flowjo.com/
Vectra 3.0
Perkin Elmer
www.perkinelmer.com/product/
Imaris
Bitplane
http://www.bitplane.com/
UCHIME
https://www.drive5.com/
usearch/manual/uchime_algo.html
http://qiime.org/
GraphPad
https://www.graphpad.com/
Other
Poly-L-Lysine Cellware 12mm round Coverslips
Corning
Cat. #354085
U bottom 96-well tissue culture plate
Olympus
Cat # 25-221
CONTACT FOR REAGENT AND RESOURCE SHARING
Further information and requests for reagents should be directed to and will be fulfilled by the Lead Contact, S.I. Grivennikov, email:
[email protected]
EXPERIMENTAL MODELS
Mice
Using Il1r1f/f conditional allele (Bruttger et al., 2015), we generated a panel of mice in which IL-1R1 expression was ablated in colonic epithelial and cancer celIs. Il1r1f/f mice were crossed to different Cre-recombinases to get cell-specific deletion of Il1r1 gene, including CD4Cre, CX3CR1Cre, CD11bCre, LysMCre and Ly6GCre. Cre-mediated recombination of these sites resulted in a frame-shift and early termination of protein translation in exon 5. CX3CR1Cre, CD4Cre, Il17aGFP, RorcGFP and LysMCre mice were obtained from Jackson Laboratory. Apcf/f, CDX2Cre (CPC) and CDX2ERT mice have been provided by E. Fearon. Ly6GCre (‘catchup’’) mice were from Matthias Gunzer (Hasenberg et al., 2015). For further information and MGI numbers identifying aforementioned mouse strains please see Key Resources Table.