U0126 Ordered from IDT mouseprimerdepot nci nih gov br

Ordered from IDT mouseprimerdepot.nci.nih.gov
RORc REV: TGCAGGAGTAGGCCACATTACA Ordered from IDT mouseprimerdepot.nci.nih.gov
IL17F FW: CCTCCCCTGGAGGATAACAC Ordered from IDT mouseprimerdepot.nci.nih.gov
IL17F REV: CATGGGGAACTGGAGCGGTT Ordered from IDT mouseprimerdepot.nci.nih.gov
Ptgs2 (COX2) FW: TGAGCAACTATTCCAAACCAGC Ordered from IDT mouseprimerdepot.nci.nih.gov
Ptgs2 (COX2) REV: GCACGTAGTCTTCGATCACTATC Ordered from IDT mouseprimerdepot.nci.nih.gov
16S bacterial RNA FW: AGAGTTTGATCMTGGCTCAG Ordered from IDT
16S bacterial RNA REV: TACGGYTACCTTGTTACGACTT Ordered from IDT
SFB FW: GACGCTGAGGCATGAGAGCAT Ordered from IDT
SFB REV: GACGGCACGGATTGTTATTCA Ordered from IDT
E.Coli FW: U0126 CATGCCGCGTGTATGAAGAA Ordered from IDT
E.Coli REV: CGGGTAACGTCAATGAGCAAA Ordered from IDT
Enterobacteria FW: GTGCCAGCMGCCGCGGTAA Ordered from IDT
Enterobacteria REV: GCCTCAAGGGCACAACCTCCAAG Ordered from IDT
Clostridia FW: CGCATAACGTTGAAAGATGG Ordered from IDT
Clostridia REV: CCTTGGTAGGCCGTTACCC Ordered from IDT
Bacteroides FW: GGTTCTGAGAGGAGGTCCC Ordered from IDT
Bacteroides REV: GCTGGCTCCCGTAGGAGT Ordered from IDT
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REAGENT or RESOURCE SOURCE IDENTIFIER
S100A8 FW Ordered from IDT mouseprimerdepot.nci.nih.gov
CCAATTCTCTGAACAAGTTTTCG
S100A8 REV Ordered from IDT mouseprimerdepot.nci.nih.gov
TCACCATGCCCTCTACAAGA
S100A9 FW: GTCCAGGTCCTCCATGATGT Ordered from IDT mouseprimerdepot.nci.nih.gov
S100A9 REV: GAAGGAAGGACACCCTGACA Ordered from IDT mouseprimerdepot.nci.nih.gov
Reg3b FW: ACTCCCTGAAGAATATACCCTCC Ordered from IDT mouseprimerdepot.nci.nih.gov
Reg3b REV: CGCTATTGAGCACAGATACGAG Ordered from IDT mouseprimerdepot.nci.nih.gov
Reg3g FW: TGCTGCTCTCCTGCCTGATG Ordered from IDT mouseprimerdepot.nci.nih.gov
Reg3g REV: ATAGGAGCCATAGGCACGGG Ordered from IDT mouseprimerdepot.nci.nih.gov
IL33 FW: TCCAACTCCAAGATTTCCCCG Ordered from IDT mouseprimerdepot.nci.nih.gov
IL33 REV: CATGCAGTAGACATGGCAGAA Ordered from IDT mouseprimerdepot.nci.nih.gov
IL18 FW: TCCTTGAAGTTGACGCAAGA Ordered from IDT mouseprimerdepot.nci.nih.gov
IL18 REV: TCCAGCATCAGGACAAAGAA Ordered from IDT mouseprimerdepot.nci.nih.gov
IL1F10 FW: TGTCTGAATCAGGGTCTCCC Ordered from IDT mouseprimerdepot.nci.nih.gov
IL1F10 REV: TGAAGACCAGACACTCCCAA Ordered from IDT mouseprimerdepot.nci.nih.gov
IL36a FW: GGCTTTTACAGGTTCCTTTTTG Ordered from IDT mouseprimerdepot.nci.nih.gov
IL36a REV: GATGAGCTGCCTGTTCTGC Ordered from IDT mouseprimerdepot.nci.nih.gov
IL36g FW: GTCCGGGTGTGGTAAAACAG Ordered from IDT mouseprimerdepot.nci.nih.gov
IL36g REV: GGACACCCTACTTTGCTGCT Ordered from IDT mouseprimerdepot.nci.nih.gov
IL36b FW: AGAGTATTCAAATGTGGGAACCG Ordered from IDT mouseprimerdepot.nci.nih.gov
IL36b REV: GACCCATACCATCTGTTGTGAG Ordered from IDT mouseprimerdepot.nci.nih.gov
IL36Ra FW: GCAGCTGGTTATTGTGCAGAT Ordered from IDT mouseprimerdepot.nci.nih.gov
IL36Ra REV: TGGAGCTCATGATGGTTCTG Ordered from IDT mouseprimerdepot.nci.nih.gov
IL36R (IL1RL2) FW: TCCCCGCTTTAATCTCTTCA Ordered from IDT mouseprimerdepot.nci.nih.gov
IL36R (IL1RL2) REV: ATCGGGCAGTCTGAATTGTC Ordered from IDT mouseprimerdepot.nci.nih.gov
IL18bp FW: TCTCCAGCAGTCCCAACTAAGC Ordered from IDT mouseprimerdepot.nci.nih.gov
IL18bp REV: AGGCAGTACAGGACAAGGTCAG Ordered from IDT mouseprimerdepot.nci.nih.gov
IL1R exon5 FW: TGGAAGTCTTGTGTGCCCTT Ordered from IDT This paper
IL1R exon5 REV: ACTCCGAAGAAGCTCACGTT
Ordered from IDT
This paper
Software and Algorithms
Flow Jo 9.7.6 FlowJo LLC https://www.flowjo.com/
Vectra 3.0 Perkin Elmer www.perkinelmer.com/product/
Imaris Bitplane http://www.bitplane.com/
UCHIME
https://www.drive5.com/
usearch/manual/uchime_algo.html
http://qiime.org/
GraphPad
https://www.graphpad.com/
Other
Poly-L-Lysine Cellware 12mm round Coverslips Corning Cat. #354085
U bottom 96-well tissue culture plate Olympus Cat # 25-221
CONTACT FOR REAGENT AND RESOURCE SHARING
Further information and requests for reagents should be directed to and will be fulfilled by the Lead Contact, S.I. Grivennikov, email:
[email protected]
EXPERIMENTAL MODELS
Mice
Using Il1r1f/f conditional allele (Bruttger et al., 2015), we generated a panel of mice in which IL-1R1 expression was ablated in colonic epithelial and cancer celIs. Il1r1f/f mice were crossed to different Cre-recombinases to get cell-specific deletion of Il1r1 gene, including CD4Cre, CX3CR1Cre, CD11bCre, LysMCre and Ly6GCre. Cre-mediated recombination of these sites resulted in a frame-shift and early termination of protein translation in exon 5. CX3CR1Cre, CD4Cre, Il17aGFP, RorcGFP and LysMCre mice were obtained from Jackson Laboratory. Apcf/f, CDX2Cre (CPC) and CDX2ERT mice have been provided by E. Fearon. Ly6GCre (‘catchup’’) mice were from Matthias Gunzer (Hasenberg et al., 2015). For further information and MGI numbers identifying aforementioned mouse strains please see Key Resources Table.